Iraqi Journal of Medical Sciences








Vol. 14 Issue 3 July - September / 2016
Published on website | Date : 2017-01-03 09:36:51

ANALYSIS OF SINGLE NUCLEOTIDE POLYMORPHISM RS9939609 IN FTO GENE OF OBESE MALES IN IRAQI POPULATION

Mustafa N. Jumaa, Nahi Y. Yaseen, Adil F. Shehab, Rafid M. Karim, Likaa H. Sagban


Abstract

Background: Obesity is a serious on public health. It contributes in many serious health conditions including high cholesterol, type 2 diabetes, osteoarthritis, high blood pressure, gallbladder disease, coronary heart disease, stroke, respiratory problems … etc. Polymorphism of TA (rs9939609) in fat mass and obesity associated (FTO) gene was found to be associated with obesity in children and adults according to many studies conducted on populations from Europe, America and Asia. Whether Single nucleotide polymorphism (SNP) rs9939609 is an associated with obesity in Iraqi population remains of concern.
Objective: To investigate the polymorphism of rs9939609 SNP in FTO gene and its relationship to the obese males in Iraqi population.
Methods: One hundred twenty of males were classified as obese based on body mass index (BMI) with mean age 20-50 year and fifty aged-matched healthy males as a control were included in this study. Lipid profile was estimated by using Spinreact-Сє, and an ELISA kit was used to assess the FTO level.
Results: The results showed that there are significant differences P ≤ 0.05 for AA genotype with all parameters whereas TA genotype showed significant differences with most of parameters in revers to TT genotype, which has showed no significant differences with most of parameters. The percentage of TT, TA, AA, alleles were 27.72%, 49.86%, 22.42% respectively, also an elevated of TT genotype frequency was observed in healthy compared to obese. On the other hand, the percentage of T and A allele frequency were 52.65% and 47.35% respectively. Also, an elevated in serum FTO enzyme level was observed in obese.
Conclusion: The presence of A risk allele in the Iraqi population is the cause in the incidence of obesity, which reflected its impact on the BMI and central obesity through the disturbances in lipid profile and FTO enzymes value.
Keywords: Single Nucleotide Polymorphism, rs9939609, Obesity, FTO gene.



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