Vol. 14 Issue 2 April - June / 2016
Published on website | Date : 2016-09-01 12:26:58
HUMAN PAPILLOMA VIRUS TYPES 16 AND 18 IN A SAMPLE OF IRAQIS PATIENTS PRESENTED WITH ORAL CANCER
Maysaa K Al-Malkey, Ahmed A.H. Abbas, Nahi Y. Yaseen
Background:Oral squamous cell carcinoma is the most common malignant neoplasm of oral mucosa. Human papilloma (HPV) virus cause a broad scope of diseases from benign to invasive tumors, types 16 and 18 classified as carcinogenic to humans.
Objective:To assess the occurrence rate of human papilloma virus genotypes in oral cancer patients and their association with various risk factors.
Methods:Fifty five (55) unstimulated whole saliva samples were collected from 35 histopathologically confirmed patients with oral cancer and 20 apparently healthy subjects were enrolled in this study. Genomic DNA was extracted from exfoliate cells to amplify HPV-DNA using HPV-L1 gene sequence primers by polymerase chain reaction method, the viral genotyping was performed using direct sequencing method.
Results:The mean age of patients group was 52.23±13.73 years, while in healthy subjects group was (50.55±12.5) years. Risk of smoking was highly significant with odds ratio 60.79 and a 95% confidence interval of 3.40-1086.71. However, the risk of alcoholism was significant with odds ratio 27.77 and a 95% confidence interval of 1.51-511.27. Forty-six percent (16/35) of oral cancer patients were positive for detection of HPV-DNA (P < 0.0002). The most frequent HPV genotypes in patients group was HPV-18 accounting for (31%) of cases (P < 0.05). The rate of HPV was significantly higher among younger ages (< 50 years) with P = 0.042. In addition, the rate of HPV was higher with other variables (male, tongue tissue, grade I differentiation, squamous cell carcinoma) with no significant association (P = 0.273, P = 0.739, P = 0.173, and P = 0.700 respectively).
Conclusion:Human papilloma virus types 16 and 18 may be a risk factor for oral cancer independent of alcohol and tobacco.
Keywords: OC, HPV, OSCC, PCR, Direct sequencing
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