Vol. 14 Issue 1 January - March / 2016
Published on website | Date : 2016-05-05 11:22:44
CONVENTIONAL METHODS FOR THE DIAGNOSIS OF PNEUMOCYSTIS JIROVECII IN IMMUNOCOMPROMISED IRAQI PATIENTS
Isa S. Touhali, Azhar A.F. Ibrahim, Haider N. Dawood
Background: Pneumocystis jirovecii is the causative agent of pneumocystis pneumonia, one of the most frequent and severe opportunistic infections in immunocompromised patients.
Objectives:To determine the possible implication of pneumocystis jirovecii in immunocompromised Iraqi patients with pulmonary infections, and investigate the efficiency of indirect qualitative immunofluorescence diagnosis in compared with conventional stains for the detection of this agent.
Methods: A total of 200 clinical samples from 100 immunocompromised patients (70 bronchoalveolar lavage, 21 sputum samples and 9 pleural fluids). One hundred samples from immunocompetent individuals (50 bronchoalveolar lavage, 30 sputum samples and 20 pleural fluids). Detection of pneumocystosis was done by conventional satins and indirect qualitative immunofluorescence technique.
Results:Fourteen samples gave positive results by indirect immunofluorescence monoclonal antibody test. Twelve out of 100 samples were positive by each Gomori methenamine silver, modified toluidine blue O stains and Diff-Quik stain (modified Giemsa).
Conclusion: Pneumocystis jirovecii is the fundamental opportunistic infection among immunocompromised patients. The indirect qualitative immunofluorescence method gives a promise for use as a primary method for diagnosis of pneumocystis jirovecii pneumonia or as rapid screen to exclude the presence of pneumocystis jirovecii in sputum and bronchoalveolar lavage samples.
Keywords: Pneumocystis jirovecii (carinii), pneumocystis jirovecii pneumonia, immunocompromised, Iraqi patients.
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