Iraqi Journal of Medical Sciences








Vol. 14 Issue 1 January - March / 2016
Published on website | Date : 2016-05-05 11:19:23

ASSOCIATION OF ATG16L1 T300A GENETIC VARIANT WITH H. PYLORI AND NONE H. PYLORI ATROPHIC GASTRITIS

Haider F. Ghazi


Abstract

Background: Helicobacter pylori persistence may develop atrophic gastritis, gastric ulcer or cancer.
Objectives:To determine the association between the presence of autophagy related gene 16 like 1 and thrionin 300 alanine mutation and H. pylori infection among atrophic gastritis patients.
Methods: Gastric biopsy was taken from eighty patients and tested for urease, and blood samples were taken for serum separation for detection of Anti-H. pylori IgG by ELISA and DNA extraction from whole blood were used for sequence specific primer – polymerase chain reaction for autophagy related gene 16 like 1 and thrionin 300 alanine mutation allelic discrimination.
Results:Among 40 H. pylori positive cases, the carriers of mutated allele were 62.5% compared with 36.25% in H. pylori negative cases (p = < 0.001, OR = 1.72, CI = 1.23-2.42).
Conclusions:Among Iraqi atrophic gastritis, there is an association between H. pylori infection and mutated autophagy related gene 16 like 1 and thrionin 300 alanine mutation allele. Thrionin 300 alanine may confer higher risk for infection with H. pylori.
Key words: Autophagy, H. pylori, atrophic gastritis.


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