Vol. 9 Issue 4 October - December / 2011
Published on website | Date : 2016-04-07 10:34:58
ADHERENCE OF CANDIDA ALBICANS TO UROEPITHELIAL CELLS
Abduladeem YA Al-Barrak, Ilham AM Al-Kawaz
Background:Urinary tract infection (UTI) is usually associated with multiplication of microorganisms in urinary tract followed by adhesion of these organisms to the uroepithelial cells which is considered as the first step for colonization and infection. Adhesion of Candida on the epithelium of the urinary tract stands as the first step in the pathogenesis of Candidiasis. The adhesion process is under the impact of many factors.
Objective:This study was designed to determine the susceptibility of uroepithelial cells obtained from patients with different urinary tract diseases, for adherence with Candida albicans in vitro.
Methods: Forty eight urine samples collected from women attending Medical City Hospital-Baghdad suffering from urinary tract diseases and proved to have Candida growth. Ten urine samples were collected from apparently healthy age matched females and used as a control. The uroepithelial cells of each sample have been collected and incubated with Candida albicans cells and the percentage of uroepithelial cells with an adhered Candida cells (% EC). The mean number of Candida/epithelial cells (C/E) was estimated before and after adherence assay.
Results: Klebsiella infection showed the highest percentage of uroepithelial cells with Candida (%EC), next in frequency was samples with high number of uroepithelial cells, then, Streptococcus pyogenes, Escherichia coli, pus cells, and finally samples with high number of RBCs. This study indicates that receptivity of Candida adhesion to the uroepithelial cells of urinary tract have different affinity depending on the type of modulating factor surrounding these uroepithelial cells.
Conclusion: From the obtained data it can be concluded that urine findings including bacterial growth might modulate epithelial cell surface and increase their receptivity for Candida adherence.
Key word: Adherence, Candida albicans, uroepithelial cells.
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