Vol. 13 Issue 2 April - June / 2015
Published on website | Date : 2016-03-23 19:37:15
EFFECT OF GLYCYRRHIZA GLABRA ON ANTIGEN INDUCED ARTHRITIS IN MICE MODEL
Abdulkareem H. Abd, Ban J. Qasim, Shihab A. Shihab, Jaffar O. Dawood
Background:Rheumatoid arthritis is a chronic inflammatory autoimmune disease represents the most common form of chronic inflammatory joint diseases. Glycyrrhiza glabra (G. glabra) was widely known to have several pharmacological activities, which might be beneficial in preventing and treating both acute and chronic inflammatory conditions.
Objective:To study the effect of aqueous extract of G.glabra on antigen induced arthritis model in mice.
Methods: Forty-eight male Swiss albino mice were used in this study. Group 1 arthritic mice without treatment (positive control); group 2 arthritic mice treated with G. glabra aqueous extract 750 mg/kg/day; group 3 arthritic mice treated with G. glabra aqueous extract 300 mg/kg/day and group 4 negative control (non-immunized, non-treated mice). Antigen induced arthritis was induced by Methylated bovine serum albumin in Imject Alum adjuvant. The mice were given the drug orally and the treatment was started from day 1 of the induction of arthritis until day 20. At day 20 of arthritis all mice were sacrificed and serum TNF-α was measured using ELISA technique. Biopsies of the left knee joint were taken for histopathological evaluation.
Results:The results indicate that G.glabra caused inhibition of histopathological features of antigen-induced arthritis in dose dependent manner. G.glabra also caused reduction of serum TNF-α concentration in antigen-induced arthritis model in dose dependent manner.
Conclusions: Glycyrrhiza glabra can significantly inhibit antigen-induced arthritis in mice. This effect seems to be in dose dependent manner.
Key words:Rheumatoid arthritis, G. glabra, TNF-α, antigen induced arthritis.
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