Vol. 11 Issue 4 October - December / 2013
Published on website | Date : 2016-03-29 11:28:30
ANALYSIS OF N-RAS GENE MUTATIONS AND P21N-RAS PROTEIN EXPRESSION IN IRAQI PATIENTS WITH IN AML
Nahidh K. Alwan, Raad J. Musa, Ban A. Abdul-Majeed
Background:N-RAS mutations are the most commonly detected molecular abnormalities in hematologic malignancies, especially in those of myeloid origin.
Objective:Current study aimed to determine the frequency of N-RAS mutation and its correlation with P21N-RAS protein expression in patients with acute myelogenous leukemia (AML) in Iraq.
Methods: Peripheral blood, bone marrow aspirate and biopsy samples were taken from 58 newly diagnosed AML patients (57 de novo and 1 therapy related AML) and 30 individuals with reactive bone marrow conditions were selected as a control group. Samples screened for N-RAS gene mutations using nested PCR followed by mutation sensitive digestion analysis (MSDA), and immunohistochemical analysis of P21N-RAS protein expression by using anti N-RAS monoclonal antibody.
Results:N-RAS mutations at the time of diagnosis were found in 10/58 (17.24%) and P21N-RAS expression found in 5/58 (8.62%) patients with AML. There was a significant difference (P = 0.001) in P21N-RAS expression between mutant and wild type N-RAS patients with AML. No N-RAS mutations or P21N-RAS expression detected in the control group individuals.
Conclusion:It can be suggested that there is activation of RAS-signaling cascade in AML patients, this is may support their role in molecular pathogenesis of acute leukemia. Also, there was a significant difference between N-RAS gene status and P21N-RAS protein expression in patients with AML.
Keyword: AML, N-RAS Mutation, MSDA, P21N-RAS expression, Digital analysis.
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