Vol. 12 Issue 2 April - June / 2014
Published on website | Date : 2016-03-27 11:19:56
EFFECTS OF VERAPAMIL AND OLANZAPINE IN TERMINATING PILOCARPINE- INDUCED EPILEPTIC SEIZURES IN MICE
Uday A. Hussein, Faruk H. Al-Jawad
Background:Epilepsy is one of the oldest known neurological conditions characterized by recurrent seizures.
Objective:To explore the possible antiepileptic effect of both Verapamil and Olanzapine in pilocarpine epileptic model in mice.
Methods: Fifty healthy male albino mice weighing between 30-35 gm were equally allocated into five groups (10 mice in each group) and were distributed into: normal group (without drug); distilled water group (0.1 ml); diazepam group (1mg/kg); verapamil group (20 mg/kg) and olanzapine group (10 mg/kg). All animals (except normal group) were injected with pilocarpine hydrochloride (350 mg/kg) to induce generalized tonic-clonic seizure 30 minutes after the tested drugs had been administrated. The mean onset of seizure were determined as well as the mean serum concentration of electrolytes, glutathione and malondialdehyde were measured after seizure had been induced.
Results: Pilocarpine-induced seizure at approximately 7 minutes after injection. While both verapamil and olanzapine produced highly significant increase in mean onset of seizure 16±1.549 and 13.1±1.566 respectively as compared to D.W. group, also both drugs produced highly significant changes in mean serum concentration of electrolytes, glutathione and malondialdehyde.
Conclusion: Verapamil and olanzapine had anticonvulsant activity when used at applied doses in the pilocarpine model of seizures in mice.
Key words:Epilepsy, seizure, verapamil and olanzapine.
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